凡纳滨对虾受精卵孵化和无节幼体优选技术探究

Study on hatching methods and nauplius selection of Litopenaeus vannamei

摘要:

为提高凡纳滨对虾受精卵孵化效率,改进优化了1种受精卵流水孵化装置,可有效地进行凡纳滨对虾无节幼体集约化高密度生产。流水孵化受精卵的孵化密度在1 000万~6 000万粒/m³,平均孵化率在85.0%~92.7%。流水孵化的孵化率与常规的产卵池孵化无显著差异,但孵化出的无节幼体的携菌量和损伤率、黏脏率明显低于产卵池孵化(P<0.05)；同时,为筛选优质的无节幼体,采用光诱、流水清洗等方法处理无节幼体,结果表明,流水清洗可显著降低无节幼体的损伤率、黏脏率和细菌携带量(P<0.05),有效提高无节幼体的生物安全及育苗成活率。

Abstract:

In this study,a flowing water hatching apparatus was optimized to produce Litopenaeus vannamei nauplius more effectively under intensive hatching condition.The density of fertilized egg varied from 1.0×10⁷ to 6.0×10⁷ cell/m³ and the average hatching rate ranged from 85.0% to 92.7% in this optimized hatching apparatus.Although no significant difference was observed on hatching rate when compare to normal spawning pool,the bacteria carrying capacity,damage rate and slime filth rate of nauplius were much superior in this optimized flowing water hatching apparatus(P<0.05).Besides, by applying light-induction and flowing water cleaning in nauplius production,the bacteria carrying capacity,damage rate and slime filth rate of nauplius decreased dramatically(P<0.05),indicated that the nauplius biosafety and breeding survival rate could be improved effectively.