基于16S rRNA高通量测序的江鳕肠道微生物群落结构研究

Microbial community in intestine of Lota lota based on 16S rRNA high-throughput sequencing

摘要:

为探究江鳕(Lota lota)肠道菌群结构特征,采用MiSeq 16S rRNA高通量测序技术对3龄、4龄、5龄野生江鳕的雌、雄样本进行了微生物测序和分析。结果显示,3个年龄组江鳕的肠道菌群丰度及多样性差异不显著(P＞0.05),同一年龄雌、雄江鳕间的肠道菌群丰度及多样性差异不显著(P＞0.05)。江鳕肠道内容物的优势菌门为变形菌门和厚壁菌门,与鱼类肠道中最普遍和共存的细菌类群相同。Mycoplasma(支原体属)存在于所有样品中,且为优势菌群,其功能有待进一步研究。

Abstract:

To explore the microbial community in intestine of Lota lota,the microbes in intestinal contents of male and female L.lota(age 3,4,5) were sequenced and analyzed by Illumina MiSeq PE300.The results showed that there was no significant difference in the abundance and diversity of intestinal flora of L.lota in three age groups as well as in different gender groups with the same age.Proteobacteria and Firmicutes were the dominant floras which were also the most commonly coexisting bacterial population in fish intestines.Mycoplasma was the dominant flora in all samples, but its function need further research.