基于AFLP分子标记的鸭绿江茴鱼遗传多样性分析

Analysis on genetic diversity of Thymallus arcticus yaluensis based on AFLP

摘要:

为探讨鸭绿江茴鱼群体遗传结构及分化水平,为其种质资源保护提供遗传学依据,利用扩增片段长度多态性(AFLP)方法,对分别采自吉林省内鸭绿江上游、松花江上游和临江金鲨养殖场3个群体的总计90尾鸭绿江茴鱼的遗传多样性进行了比较研究。结果显示,鸭绿江上游、松花江上游和临江金鲨养殖场3个群体的观测等位基因数(N_a)分别为1.340 9、1.251 7和1.269 1,有效等位基因数(N_e)分别为1.153 8、1.130 0和1.144 1,Nei’s遗传多样性指数分别为0.093 2、0.078 2和0.086 3,Shannon’s信息指数分别为0.144 7、0.119 8和0.131 9,遗传分化系数(G_st)为0.176 7~0.254 7,平均值为0.219 4,遗传相似性为0.820 6~0.936 3。UPGMA聚类分析结果显示,松花江上游群体单独聚为一支,鸭绿江上游群体和临江金鲨养殖场群体出现了聚群情况。结果表明,采样的3个鸭绿江茴鱼群体均具有较高的遗传多样性水平,3个群体发生了显著的遗传分化。

Abstract:

Thymallus arcticus yaluensis is the second class national key protection animal.To discuss population genetic structure and genetic differentiation level of T. arcticus yaluensis in Jilin Province,90 T. arcticus yaluensis collected from the upstream of Yalu River,the upstream of Songhua River and Jinsha Farm of Linjiang were used to investigate the genetic diversity by amplified fragment length polymorphism(AFLP) method.The results showed that the number of observed allele(N_a) of T. arcticus yaluensis collected from the upstream of Yalu River,the upstream of Songhua River and Jinsha Farm of Linjiang were 1.340 9,1.251 7 and 1.269 1,respectively;the number of effective allele(N_e) were 1.153 8,1.130 0 and 1.144 1,respectively;the Nei diversity index were 0.093 2,0.078 2 and 0.086 3,respectively;the Shannon’s information index were 0.144 7,0.119 8 and 0.131 9,respectively.The genetic differentiation coefficient(G_st) was 0.176 7-0.254 7 with the mean value of 0.219 4,and the genetic similarity was 0.820 6-0.936 3.UPGMA clustering revealed that the Songhua River population was clustered into a single group,but the populations of Yalu River and Jinsha Farm of Linjiang were clustered into a nother aggregation.This research indicated that three populations of T. arcticus yaluensis in Jilin Province had high levels of genetic diversity and a significant genetic differentiation.