头孢噻肟钠对鲟源嗜水气单胞菌和弗氏柠檬酸杆菌的体外药效学研究

Pharmacodynamic study of cefotaxime sodium on Aeromonas hydrophila and Citrobacter freundii from sturgeon in vitro

摘要:

文章旨在筛选出一种针对中华鲟嗜水气单胞菌和弗氏柠檬酸杆菌两个细菌性疾病的治疗药物并为其使用提供理论参考。本研究针对鲟源嗜水气单胞菌和弗氏柠檬酸杆菌进行了药物敏感性试验,并测定头孢噻肟钠对两种菌株的最小抑菌浓度(MIC),建立1 MIC、2 MIC、4 MIC、8 MIC浓度下头孢噻肟钠对两种菌株的杀菌动力学曲线,测定头孢噻肟钠对两种细菌的抗菌后效应(PAE)。药敏试验结果表明两种菌株均对四种头孢菌素敏感或中度敏感,对多粘菌素B、复方新诺明、庆大霉素、红霉素、氟苯尼考、恩诺沙星等九种药物存在不同程度的耐药。头孢噻肟钠对弗氏柠檬酸杆菌和嗜水气单胞菌的MIC均为1 mg/L。杀菌动力学曲线表明,头孢噻肟钠对两种菌株的抑菌效果表现出强时间依赖性及浓度依赖性。PAE研究表明2 MIC、4 MIC、8 MIC浓度的头孢噻肟钠对弗氏柠檬酸杆菌的体外PAE (h)分别为0.22 ± 0.12、0.39 ± 0.09、0.67 ± 0.2,对嗜水气单胞菌的体外PAE (h)为1.24 ± 0.17、2.64 ± 0.31、2.96 ± 0.28。本研究结果证明头孢噻肟钠对鲟源嗜水气单胞菌与弗氏柠檬酸杆菌具有较好的体外抑制效果。在抗生素耐药问题日益严重的背景下，头孢噻肟钠作为非国标渔药，目前不能在养殖过程中使用，但本研究可作为一种储备，可为新渔药开发做为基础。

Abstract:

The purpose of this paper is to screen out a therapeutic drug for two bacterial diseases of ACIPENSER sinensis, Aeromonas hydrophila and Citrobacter freundii, and to provide theoretical reference for its use. In this study, drug sensitivity tests were carried out against Aeromonas hydrophila and Citrobacter freundii from sturgeon, and the minimum inhibitory concentration (MIC) of cefotaxime sodium to the two strains was determined. The bactericidal kinetic curves of cefotaxime sodium to the two strains were established at the concentrations of 1 MIC, 2 MIC, 4 MIC and 8 MIC, and the post antibiotic effect (PAE) of cefotaxime sodium to the two strains was determined. The results of drug sensitivity test showed that the two strains were sensitive to four cephalosporins or moderately sensitive, and they were resistant to nine drugs, such as polymyxin B, cotrimoxazole, gentamicin, erythromycin, florfenicol and enrofloxacin, to varying degrees. The MIC of cefotaxime sodium to Citrobacter freundii and Aeromonas hydrophila was 1 mg/L. The bactericidal kinetic curve showed that the antibacterial effect of cefotaxime sodium on the two strains showed strong time and concentration dependence. PAE study showed that the in vitro PAE (h) of cefotaxime sodium at 2 MIC, 4 MIC and 8 MIC were 0.22 0.12, 0.39 0.09 and 0.67 0.2 for Citrobacter freundii, and 1.24 0.17, 2.64 0.31, respectively for Aeromonas hydrophila. The results of this study proved that cefotaxime sodium had a good inhibitory effect on Aeromonas hydrophila and Citrobacter freundii from sturgeon in vitro. Against the background of increasingly serious antibiotic resistance, cefotaxime sodium, as a non-national fishery drug, can not be used in aquaculture at present, but this study can be used as a reserve and as a basis for the development of new fishery drugs.