Abstract:In the seawater recirculating aquaculture system, the biofilm formation time of moving-bed biofilm reactor(MBBR) is usually long.By using experimental strategies such as stirring filter,low-cost seawater formula,specialized nutrient solution and diversified bacterial strain selection,the rapid biofilm formation of MBBR filter under artificial seawater conditions was achieved,and an evaluation system based on ammonia nitrogen,nitrite nitrogen level,filter color,and pore products of filter was established.The fermentation nutrition group started up in 46 days,while the chemical nutrition group started up in 56 days.During the start-up process,the highest concentration of ammonia and nitrite was 20.36-25.12 mg/L and 45.28-53.20 mg/L,respectively.