Abstract:[Objective] This experiment was conducted to investigate the effects of dietary different supplementation on the growth, non-specific immunity, metabolic ability and intestinal tissue structure of Litopenaeus vannamei (L. vannamei) in the industrial aquaculture. [Method ] This study focused on 2 250 L. vannamei with an initial body weight of (2±0.2) g and randomly divided them into 3 groups, each group with 3 replicates, and each replicate with 250 L. vannamei. The control diet contained unsupplemental basic feed, the experimental diets 1 contained peptides+amino acids+Bacillus, the experimental diets 2 contained Allium sativum L. + Cinnamomum cassia(L.) + Cichorium intybus L.extracts + Bacillus, the addition amount was 3 g/kg basic feed. After a 30 day feeding trial, the significance of supplemental diets was evaluated through changes in shrimp growth indicators, muscle nutrition, non-specific immune enzyme and metabolic enzyme activity in serum, and intestinal tissue structure. [Results] The results showed that the supplemental diets could significantly increase the weight gain rate and specific growth rate of shrimp (P<0.05), and supplemental diet 2 was better. The supplemental diets could increase the crude protein and flavor amino acid content, with supplemental diet 1 having a better effect (P<0.05). The activities of antioxidant enzymes such as superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LSZ) and nitricoxidesynthase (NOS) in shrimp serum showed an increasing trend compared to the control group,gtoup 2 showed a significant difference (P<0.05); but the activities of glutathione peroxidase (GSH-Px), alkaline phosphatase (AKP) and acid phosphatase (ACP) decreased (P<0.05). The results of metabolic enzyme activity showed that the activity of glutamine synthase (GS) was increased with a significant difference in supplemental diet 1 (P<0.05). The activity of malate dehydrogenase (MDH) showed a decreasing trend, and there was no significant difference (P>0.05). There was no significant difference in hexokinase (HK) compared to the control group (P<0.05). The HE results of intestinal tissue showed that the supplemental diets could improve the structure arrangement of intestinal tissue, reduce the shedding of villous layer, and has a positive effect on promoting intestinal health of shrimp. [Conclusion] The 3 g/kg supplemental diets can promote the growth, improve activities of non-specific immunoenzymes and metabolic abilities, optimize intestinal tissue structure of L. vannamei, indirectly improve shrimp immunity, and provide theoretical basis for disease prevention and healthy aquaculture.